Thermal damage threshold of neurons during infrared stimulation
| dc.contributor.author | Brown, William | |
| dc.contributor.author | Needham, Karina | |
| dc.contributor.author | Begeng, James | |
| dc.contributor.author | Thompson, Alexander | |
| dc.contributor.author | Nayagam, Bryony | |
| dc.contributor.author | Kameneva, Tatiana | |
| dc.contributor.author | Stoddart, Paul | |
| dc.date.accessioned | 2020-04-29T06:00:49Z | |
| dc.date.available | 2020-04-29T06:00:49Z | |
| dc.date.issued | 2020-04 | |
| dc.description.abstract | In infrared neural stimulation (INS), laser-evoked thermal transients are used to generate small depolarising currents in neurons. The laser exposure poses a moderate risk of thermal damage to the target neuron. Indeed, exogenous methods of neural stimulation often place the target neurons under stressful non-physiological conditions, which can hinder ordinary neuronal function and hasten cell death. Therefore, quantifying the exposure-dependent probability of neuronal damage is essential for identifying safe operating limits of INS and other interventions for therapeutic and prosthetic use. Using patch-clamp recordings in isolated spiral ganglion neurons, we describe a method for determining the dose-dependent damage probabilities of individual neurons in response to both acute and cumulative infrared exposure parameters based on changes in injection current. The results identify a local thermal damage threshold at approximately 60 °C, which is in keeping with previous literature and supports the claim that damage during INS is a purely thermal phenomenon. In principle this method can be applied to any potentially injurious stimuli, allowing for the calculation of a wide range of dose-dependent neural damage probabilities. Unlike histological analyses, the technique is well-suited to quantifying gradual neuronal damage, and critical threshold behaviour is not required. | en_US |
| dc.description.sponsorship | Australian Research Council Linkage Project (LP120100264). | en_US |
| dc.identifier.citation | Brown, W. G. A., K. Needham, J. M. Begeng, A. C. Thompson, B. A. Nayagam, T. Kameneva, and P. R. Stoddart. 2020. Thermal damage threshold of neurons during infrared stimulation. Biomedical Optics Express. 11(4): 2224-2234. | en_US |
| dc.identifier.issn | 1741-2552 | |
| dc.identifier.uri | http://repository.bionicsinstitute.org:8080/handle/123456789/396 | |
| dc.language.iso | en | en_US |
| dc.publisher | Biomedical Optics Express | en_US |
| dc.title | Thermal damage threshold of neurons during infrared stimulation | en_US |
| dc.type | Article | en_US |