Retinal Changes in an ATP-Induced Model of Retinal Degeneration

Simple item page
dc.contributor.authorAplin, Felix
dc.contributor.authorVessey, Kirstan
dc.contributor.authorLuu, Chi
dc.contributor.authorGuymer, Robyn
dc.contributor.authorShepherd, Robert
dc.contributor.authorFletcher, Erica
dc.date.accessioned2017-09-06T06:01:56Z
dc.date.available2017-09-06T06:01:56Z
dc.date.issued2016-04
dc.description.abstractIn rodents and felines, intravitreal administration of adenosine triphosphate (ATP) has been shown to induce photoreceptor death providing a tractable model of retinal degeneration in these species. This study investigated the long term effects of photoreceptor loss in an ATP induced feline model of retinal degeneration. Six normal sighted felines were unilaterally blinded using intravitreal ATP injections and assessed using electroretinography (ERG) and optical coherence tomography (OCT). At 30 h (n = 3) or 12 weeks (n = 3) post-injection, the animals were euthanized and the eyes enucleated. Retinae were sectioned and labeled using immunohistochemistry for markers of cell death, neural remodeling and gliosis. Ongoing cell death and retinal degeneration was observed in the outer retina at both 30 h and 12 weeks following unilateral ATP injection. Markers of mid to late-stage retinal remodeling such as cell displacement and aberrant neurite growth were observed in the inner retina at 12 weeks post-injection. Ganglion cells appeared to remain intact in ATP injected eyes. Müller cell gliosis was observed throughout the inner and outer retina, in some parts completely enveloping and/or displacing the surviving neural tissue. Our data suggests that the ATP injected feline retina continues to undergo progressive retinal degeneration and exhibits abnormalities consistent with a description of retinal remodeling commonly seen in other models of retinal degeneration. These findings validate the use of intravitreal ATP injection in feline as a large animal model of retinal degeneration which may aid in development of therapies aiming to restore visual function after photoreceptor degeneration.en_US
dc.description.sponsorshipThis work was supported by the Australian Research Council (ARC) through its Special Research Initiative(SRI)in Bionic Vision Science and Technology grant to Bionic Vision Australia (BVA) and by the National Health and Medical Research Council (NHMRC)project grant(APP1021042;APP1061419) to ELF by Retina Australia.The Bionics Institute and CERA receive Operational Infrastructure Support from the Victorian Government.en_US
dc.identifier.citationAplin, F., K. Vessey, C. D. Luu, R. Guymer, R. K. Shepherd, and E. L. Fletcher. 2016. Retinal Changes in an ATP-Induced Model of Retinal Degeneration. Frontiers in neuroanatomy. 10: 46.en_US
dc.identifier.urihttp://repository.bionicsinstitute.org:8080/handle/123456789/262
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.subjectRetinaen_US
dc.subjectGliosisen_US
dc.subjectAnimal modelsen_US
dc.subjectAdenosine triphosphateen_US
dc.subjectRetinal degenerationen_US
dc.subjectFelineen_US
dc.subjectPhotoreceptor cellsen_US
dc.subjectRetinitis pigmentosaen_US
dc.titleRetinal Changes in an ATP-Induced Model of Retinal Degenerationen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2016_Aplin_Retinalchanges.pdf
Size:
4.57 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
BI publications