Implantation Surgery for Abdominal Vagus Nerve Stimulation and Recording Studies in Awake Rats.

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dc.contributor.authorHyakumura, Tomoko
dc.contributor.authorFallon, James B
dc.contributor.authorPayne, Sophie C
dc.date.accessioned2024-02-06T22:58:31Z
dc.date.available2024-02-06T22:58:31Z
dc.date.issued2024-01-19
dc.description.abstractAbdominal vagus nerve stimulation (VNS) can be applied to the subdiaphragmatic branch of the vagus nerve of rats. Due to its anatomical location, it does not have any respiratory and cardiac off-target effects commonly associated with cervical VNS. The lack of respiratory and cardiac off-target effects means that the intensity of stimulation does not need to be lowered to reduce side effects commonly experienced during cervical VNS. Few recent studies demonstrate the anti-inflammatory effects of abdominal VNS in rat models of inflammatory bowel disease, rheumatoid arthritis, and glycemia reduction in a rat model of type 2 diabetes. Rat is a great model to explore the potential of this technology because of the well-established anatomy of the vagus nerve, the large size of the nerve that allows easy handling, and the availability of many disease models. Here, we describe the methods for cleaning and sterilizing the abdominal VNS electrode array and surgical protocol in rats. We also describe the technology required for confirmation of suprathreshold stimulation by recording evoked compound action potentials. Abdominal VNS has the potential to offer selective, effective treatment for a variety of conditions, including inflammatory diseases, and the application is expected to expand similarly to cervical VNS.
dc.description.sponsorshipDevelopment of the rat abdominal VNS implant was funded by the Defense Advanced Research Projects Agency (DARPA) BTO, under the auspices of Dr. Doug Weber and Dr. Eric Van Gieson through the Space and Naval Warfare Systems Center (Contract No. N66001-15-2-4060). Research reported in this publication was supported by the Bionics Institute Incubation Fund. The Bionics Institute acknowledges the support they receive from the Victorian Government through its Operational Infrastructural Support Program. We would like to thank Mr. Owen Burns for mechanical design, Prof. John B Furness for anatomical expertise, Prof. Robert K Shepherd for peripheral interface, neuromodulation and recording expertise, Ms. Philippa Kammerer and Ms. Amy Morley for animal husbandry and testing, Ms. Fenella Muntz and Dr. Peta Grigsby for their advice on post-operative animal care, and Ms. Jenny Zhou and the electrode fabrication team from NeoBionica for production of the VNS arrays.
dc.identifier.citationHyakumura, T., Fallon, J.B., Payne, S.C. Implantation Surgery for Abdominal Vagus Nerve Stimulation and Recording Studies in Awake Rats. J. Vis. Exp. (203), e65896, doi:10.3791/65896 (2024).
dc.identifier.urihttps://repository.bionicsinstitute.org/handle/123456789/430
dc.language.isoen
dc.publisherJournal of Visualized Experiments
dc.titleImplantation Surgery for Abdominal Vagus Nerve Stimulation and Recording Studies in Awake Rats.
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