Combined optogenetic and electrical stimulation of auditory neurons increases effective stimulation frequency—an in vitro study

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dc.contributor.authorHart, William
dc.contributor.authorRichardson, Rachael
dc.contributor.authorKameneva, Tatiana
dc.contributor.authorThompson, Alex
dc.contributor.authorWise, Andrew
dc.contributor.authorFallon, James
dc.contributor.authorStoddart, Paul
dc.contributor.authorNeedham, Karina
dc.date.accessioned2020-04-29T05:42:24Z
dc.date.available2020-04-29T05:42:24Z
dc.date.issued2020-01
dc.description.abstractOBJECTIVE: The performance of neuroprostheses, including cochlear and retinal implants, is currently constrained by the spatial resolution of electrical stimulation. Optogenetics has improved the spatial control of neurons in vivo but lacks the fast-temporal dynamics required for auditory and retinal signalling. The objective of this study is to demonstrate that combining optical and electrical stimulation in vitro could address some of the limitations associated with each of the stimulus modes when used independently. APPROACH: The response of murine auditory neurons expressing ChR2-H134 to combined optical and electrical stimulation was characterised using whole cell patch clamp electrophysiology. MAIN RESULTS: Optogenetic costimulation produces a three-fold increase in peak firing rate compared to optical stimulation alone and allows spikes to be evoked by combined subthreshold optical and electrical inputs. Subthreshold optical depolarisation also facilitated spiking in auditory neurons for periods of up to 30 ms without evidence of wide-scale Na+ inactivation. Significance These findings may contribute to the development of spatially and temporally selective optogenetic-based neuroprosthetics and complement recent developments in "fast opsins".en_US
dc.description.sponsorshipThis work is supported by Action on Hearing Loss International project grant G89 and the ARC Training Centre in Biodevices (IC140100023). William Hart is supported by an Australian Government Research Training Program (RTP) Scholarship.en_US
dc.identifier.citationHart, W., R. Richardson, T. Kameneva, A. Thompson, A. K. Wise, J. B. Fallon, P. R. Stoddart, and K. Needham. 2020. Combined optogenetic and electrical stimulation of auditory neurons increases effective stimulation frequency - An in vitro study. Journal of Neural Engineering. 17(1): 016069.en_US
dc.identifier.issn1741-2552
dc.identifier.urihttp://repository.bionicsinstitute.org:8080/handle/123456789/395
dc.language.isoenen_US
dc.publisherIOP Publishingen_US
dc.subjectOptogeneticsen_US
dc.subjectNeural stimulationen_US
dc.subjectNeuroprosthesisen_US
dc.subjectOptical stimulationen_US
dc.subjectChannelrhodopsinen_US
dc.subjectElectrical stimulationen_US
dc.subjectPatch clamp electrophysiologyen_US
dc.titleCombined optogenetic and electrical stimulation of auditory neurons increases effective stimulation frequency—an in vitro studyen_US
dc.typeArticleen_US
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