Comparing Perilymph Proteomes Across Species

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dc.contributor.authorPalmer, Jonathon
dc.contributor.authorLord, Megan
dc.contributor.authorPinyon, Jeremy
dc.contributor.authorWise, Andrew
dc.contributor.authorLovell, Nigel
dc.contributor.authorCarter, Paul
dc.contributor.authorEnke, Ya Lang
dc.contributor.authorHousley, Gary
dc.contributor.authorGreen, Rylie
dc.date.accessioned2019-01-07T03:17:06Z
dc.date.available2019-01-07T03:17:06Z
dc.date.issued2018-01
dc.description.abstractOBJECTIVES/HYPOTHESIS: Biological components of perilymph affect the electrical performance of cochlear implants. Understanding the perilymph composition of common animal models will improve the understanding of this impact and improve the interpretation of results from animal studies and how it relates to humans. STUDY DESIGN: Analysis and comparison of the proteomes of human, guinea pig, and cat perilymph. METHODS: Multiple perilymph samples from both guinea pigs and cats were analysed via liquid chromatography with tandem mass spectrometry. Proteins were identified using the Mascot database. Human data were obtained from a published dataset. Proteins identified were refined to form a proteome for each species. RESULTS: Over 200 different proteins were found per species. There were 81, 39, and 64 proteins in the final human, guinea pig, and cat proteomes, respectively. Twenty-one proteins were common to all three species. Fifty-two percent of the cat proteome was found in the human proteome, and 31% of the guinea pig was common to human. The cat proteome had similar complexity to the human proteome in three protein classes, whereas the guinea pig had a similar complexity in two. The presence of albumin was significantly higher in human perilymph than in the other two species. Immunoglobulins were more abundant in the human than in the cat proteome. CONCLUSIONS: Perilymph proteomes were compared across three species. The degree of crossover of proteins of both guinea pig and cat with human indicate that these animals suitable models for the human cochlea, albeit the cat perilymph is a closer match. LEVEL OF EVIDENCE: NA. Laryngoscope, 128:E47-E52, 2018.en_US
dc.description.sponsorshipThis research supported by ARC Linkage grant LP140100109 and Cochlear Ltd. A.K.W. is funded by the Australian National Health and Medical Research Council (GNT 1064375). Bionics Institute acknowledges the support it receives from the Victorian Government through its Operational Infrastructure Support Program. Mass spectrometric analysis for this work was carried out at the Bioanalytical Mass Spectrometry Facility, University of New South Wales, and was supported in part by infrastructure funding from the New South Wales government as part of its coinvestment in the National Collaborative Research Infrastructure Strategy.en_US
dc.identifier.citationPalmer, J. C., M. S. Lord, J. L. Pinyon, A. K. Wise, N. H. Lovell, P. M. Carter, Y. L. Enke, G. D. Housley, and R. A. Green. 2018. Comparing perilymph proteomes across species. Laryngoscope. 128(1): E47-e52.en_US
dc.identifier.issn0023-852x
dc.identifier.urihttp://repository.bionicsinstitute.org:8080/handle/123456789/330
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectPerilymph proteomeen_US
dc.subjectHumanen_US
dc.subjectGuinea pigen_US
dc.subjectCaten_US
dc.subjectCochlear implanten_US
dc.subjectimpedanceen_US
dc.titleComparing Perilymph Proteomes Across Speciesen_US
dc.typeArticleen_US
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