Combined-electrical optogenetic stimulation but not channelrhodopsin kinetics improves the fidelity of high rate stimulation in the auditory pathway in mice.
| dc.contributor.author | Ajay, Elise A | |
| dc.contributor.author | Thompson, Alex C | |
| dc.contributor.author | Azees, Ajmal A | |
| dc.contributor.author | Wise, Andrew K | |
| dc.contributor.author | Grayden, David B | |
| dc.contributor.author | Fallon, James B | |
| dc.contributor.author | Richardson, Rachael T | |
| dc.date.accessioned | 2024-09-12T04:36:54Z | |
| dc.date.available | 2024-09-12T04:36:54Z | |
| dc.date.issued | 2024-09-09 | |
| dc.description.abstract | Novel stimulation methods are needed to overcome the limitations of contemporary cochlear implants. Optogenetics is a technique that confers light sensitivity to neurons via the genetic introduction of light-sensitive ion channels. By controlling neural activity with light, auditory neurons can be activated with higher spatial precision. Understanding the behaviour of opsins at high stimulation rates is an important step towards their translation. To elucidate this, we compared the temporal characteristics of auditory nerve and inferior colliculus responses to optogenetic, electrical, and combined optogenetic-electrical stimulation in virally transduced mice expressing one of two channelrhodopsins, ChR2-H134R or ChIEF, at stimulation rates up to 400 pulses per second (pps). At 100 pps, optogenetic responses in ChIEF mice demonstrated higher fidelity, less change in latency, and greater response stability compared to responses in ChR2-H134R mice, but not at higher rates. Combined stimulation improved the response characteristics in both cohorts at 400 pps, although there was no consistent facilitation of electrical responses. Despite these results, day-long stimulation (up to 13 h) led to severe and non-recoverable deterioration of the optogenetic responses. The results of this study have significant implications for the translation of optogenetic-only and combined stimulation techniques for hearing loss. | |
| dc.description.sponsorship | We gratefully acknowledge Ella P. Trang and Philippa Kammerer for their histological expertise and research support. Te authors acknowledge our funding sources: the National Health and Medical Research Council (NHMRC; #2002523), the Australian Government Research Training Program, the Bionics Institute Incubator Fund, and the Graeme Clark Institute for Biomedical Engineering. Te Bionics Institute acknowledges the support it receives from the Victorian Government through the operational infrastructure program. | |
| dc.identifier.citation | Ajay EA, Thompson AC, Azees AA, Wise AK, Grayden DB, Fallon JB, Richardson RT. Combined-electrical optogenetic stimulation but not channelrhodopsin kinetics improves the fidelity of high rate stimulation in the auditory pathway in mice. Sci Rep. 2024 Sep 9;14(1):21028. doi: 10.1038/s41598-024-71712-9. PMID: 39251630. | |
| dc.identifier.uri | https://repository.bionicsinstitute.org/handle/123456789/463 | |
| dc.language.iso | en | |
| dc.publisher | Scientific Reports | |
| dc.title | Combined-electrical optogenetic stimulation but not channelrhodopsin kinetics improves the fidelity of high rate stimulation in the auditory pathway in mice. | |
| dc.type | Article |