Promoting neurite outgrowth from spiral ganglion neuron explants using polypyrrole/BDNF-coated electrodes

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dc.contributor.authorEvans, Alison
dc.contributor.authorThompson, Brianna
dc.contributor.authorWallace, Gordon
dc.contributor.authorMillard, Rodney
dc.contributor.authorO'Leary, Stephen
dc.contributor.authorClark, Graeme
dc.contributor.authorShepherd, Robert
dc.contributor.authorRichardson, Rachael
dc.date.accessioned2014-01-28T05:30:14Z
dc.date.available2014-01-28T05:30:14Z
dc.date.issued2009-10
dc.description.abstractRelease of neurotrophin-3 (NT3) and brain-derived neurotrophic factor (BDNF) from hair cells in the cochlea is essential for the survival of spiral ganglion neurons (SGNs). Loss of hair cells associated with a sensorineural hearing loss therefore results in degeneration of SGNs, potentially reducing the performance of a cochlear implant. Exogenous replacement of either or both neurotrophins protects SGNs from degeneration after deafness. We previously incorporated NT3 into the conducting polymer polypyrrole (Ppy) synthesized with para-toluene sulfonate (pTS) to investigate whether Ppy/pTS/NT3-coated cochlear implant electrodes could provide both neurotrophic support and electrical stimulation for SGNs. Enhanced and controlled release of NT3 was achieved when Ppy/pTS/NT3-coated electrodes were subjected to electrical stimulation. Here we describe the release dynamics and biological properties of Ppy/pTS with incorporated BDNF. Release studies demonstrated slow passive diffusion of BDNF from Ppy/pTS/BDNF, with electrical stimulation significantly enhancing BDNF release over seven days. A three-day SGN explant assay found neurite outgrowth from explants was 12.3-fold greater when polymers contained BDNF (p<0.001), although electrical stimulation did not increase neurite outgrowth further. The versatility of Ppy to store and release neurotrophins, conduct electrical charge and act as a substrate for nerve-electrode interactions is discussed for specialized applications such as cochlear implants.en_US
dc.description.sponsorshipThe authors would like to thank the following funding institutions associated with this research: The University of Melbourne, Department of Otolaryngology, Stavros S. Niarchos Foundation, John T Reid Charitable Trusts, Royal National Institute for Deaf People, Pierce Armstrong Foundation and Australian Research Council Centre of Excellence for Electromaterials Science.en_US
dc.identifier.citationEvans AJ, Thompson BC, Wallace GG, Millard R, O’Leary SJ, Clark GM, Shepherd RK & Richardson RT (2008). Promoting neurite outgrowth from spiral ganglion neuron explants using polypyrrole/BDNF-coated electrodes. Journal of Biomedical Materials Research Part A 91 (1):241-50.en_US
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1002/jbm.a.32228/abstract;jsessionid=302E7DF521E9412DBAAF6F50513D1B56.f02t04?deniedAccessCustomisedMessage=&userIsAuthenticated=false
dc.identifier.urihttp://repository.bionicsinstitute.org:8080/handle/123456789/65
dc.language.isoenen_US
dc.publisherWiley Periodicalsen_US
dc.subjectBrain Derived Neurotrophic Factoren_US
dc.subjectPolypyrroleen_US
dc.subjectCochlear Implanten_US
dc.subjectElectrical Stimulationen_US
dc.subjectSensorineural Hearing Lossen_US
dc.subjectSpiral Ganglion Neuronen_US
dc.titlePromoting neurite outgrowth from spiral ganglion neuron explants using polypyrrole/BDNF-coated electrodesen_US
dc.typeArticleen_US
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