Generation of Vestibular Tissue-Like Organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture System

Simple item page
dc.contributor.authorMattei, Cristiana
dc.contributor.authorLim, Rebecca
dc.contributor.authorDrury, Hannah
dc.contributor.authorNasr, Babak
dc.contributor.authorLi, Zihui
dc.contributor.authorTadros, Melissa
dc.contributor.authorD'Abaco, Giovanna
dc.contributor.authorStok, Kathyrn
dc.contributor.authorNayagam, Bryony
dc.contributor.authorDottori, Mirella
dc.date.accessioned2019-08-27T06:16:03Z
dc.date.available2019-08-27T06:16:03Z
dc.date.issued2019-03
dc.description.abstractHair cells are specialized mechanosensitive cells responsible for mediating balance and hearing within the inner ear. In mammals, hair cells are limited in number and do not regenerate. Human pluripotent stem cells (hPSCs) provide a valuable source for deriving human hair cells to study their development and design therapies to treat and/or prevent their degeneration. In this study we used a dynamic 3D Rotary Cell Culture System (RCCS) for deriving inner ear organoids from hPSCs. We show RCCS-derived organoids recapitulate stages of inner ear development and give rise to an enriched population of hair cells displaying vestibular-like morphological and physiological phenotypes, which resemble developing human fetal inner ear hair cells as well as the presence of accessory otoconia-like structures. These results show that hPSC-derived organoids can generate complex inner ear structural features and be a resource to study inner ear development.en_US
dc.description.sponsorshipThis study was supported by the University of Melbourne and Melbourne International Research Scholarship (CM), Australian Research Council Future Fellowship (MD; FT130100514), Garnett Passe and Rodney William Memorial Foundation.en_US
dc.identifier.citationMattei, C., R. Lim, H. Drury, B. Nasr, Z. Li, M. A. Tadros, G. M. D'Abaco, K. S. Stok, B. A. Nayagam, and M. Dottori. 2019. Generation of Vestibular Tissue-Like Organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture System. Front Cell Dev Biol. 7: 25.en_US
dc.identifier.issn2296-634x
dc.identifier.urihttp://repository.bionicsinstitute.org:8080/handle/123456789/366
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.subjectVestibular hair cellsen_US
dc.subjectHuman pluripotent stem cellsen_US
dc.subjectOrganoidsen_US
dc.subjectHuman fetal tissueen_US
dc.subjectInner earen_US
dc.titleGeneration of Vestibular Tissue-Like Organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture Systemen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2019_Mattei_Generation.pdf
Size:
5.55 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
BI publications