Changes in ganglion cells during retinal degeneration

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dc.contributor.authorSaha, Susmita
dc.contributor.authorGreferath, Ursula
dc.contributor.authorVessey, Kirstan
dc.contributor.authorGrayden, David
dc.contributor.authorBurkitt, Anthony
dc.contributor.authorFletcher, Erica
dc.date.accessioned2017-09-06T05:31:06Z
dc.date.available2017-09-06T05:31:06Z
dc.date.issued2016-04
dc.description.abstractInherited retinal degeneration such as retinitis pigmentosa (RP) is associated with photoreceptor loss and concomitant morphological and functional changes in the inner retina. It is not known whether these changes are associated with changes in the density and distribution of synaptic inputs to retinal ganglion cells (RGCs). We quantified changes in ganglion cell density in rd1 and age-matched C57BL/6J-(wildtype, WT) mice using the immunocytochemical marker, RBPMS. Our data revealed that following complete loss of photoreceptors, (approximately 3months of age), there was a reduction in ganglion cell density in the peripheral retina. We next examined changes in synaptic inputs to A type ganglion cells by performing double labeling experiments in mice with the ganglion cell reporter lines, rd1-Thy1 and age-matched wildtype-Thy1. Ribbon synapses were identified by co-labelling with CtBP2 (RIBEYE) and conventional synapses with the clustering molecule, gephyrin. ON RGCs showed a significant reduction in RIBEYE-immunoreactive synapse density while OFF RGCs showed a significant reduction in the gephyrin-immmunoreactive synapse density. Distribution patterns of both synaptic markers across the dendritic trees of RGCs were unchanged. The change in synaptic inputs to RGCs was associated with a reduction in the number of immunolabeled rod bipolar and ON cone bipolar cells. These results suggest that functional changes reported in ganglion cells during retinal degeneration could be attributed to loss of synaptic inputs.en_US
dc.description.sponsorshipThis work was supported by the National Health and Medical Research Council (Australia) project grant (APP1021042), by Retina Australia, and the Australian Research Council, through its Special Research Initiative in Bionic Vision Science and Technology grant to Bionic Vision Australia.en_US
dc.identifier.citationSaha, S., U. Greferath, K. A. Vessey, D. B. Grayden, A. N. Burkitt, and E. L. Fletcher. 2016. Changes in ganglion cells during retinal degeneration. Neuroscience. 329: 1-11.en_US
dc.identifier.issn1873-7544 (Electronic) 0306-4522 (Linking)
dc.identifier.urihttp://repository.bionicsinstitute.org:8080/handle/123456789/261
dc.language.isoenen_US
dc.publisherIOP Publishingen_US
dc.subjectGanglion cellen_US
dc.subjectRetinitis pigmentosaen_US
dc.subjectPlasticityen_US
dc.subjectRetinaen_US
dc.subjectInner plexiform layeren_US
dc.titleChanges in ganglion cells during retinal degenerationen_US
dc.typeArticleen_US
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