Transmural impedance detects graded changes of inflammation in experimental colitis

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dc.contributor.authorPayne, Sophie
dc.contributor.authorAlexandrovics, Jack
dc.contributor.authorThomas, Ross
dc.contributor.authorShepherd, Robert
dc.contributor.authorFurness, John
dc.contributor.authorFallon, James
dc.date.accessioned2020-02-24T02:47:11Z
dc.date.available2020-02-24T02:47:11Z
dc.date.issued2020-02
dc.description.abstractUlcerative colitis is a chronic disease in which the mucosa of the colon or rectum becomes inflamed. An objective biomarker of inflammation will provide quantitative measures to support qualitative assessment during an endoscopic examination. Previous studies show that transmural electrical impedance is a quantifiable biomarker of inflammation. Here, we hypothesize that impedance detects spatially restricted areas of inflammation, thereby allowing the distinction between regions that differ in their severity of inflammation. A platinum ball electrode was placed into minimally inflamed (i.e. normal) or 2,4,6-trinitrobenzene sulphonic acid (TNBS)-inflamed colonic regions of rats and impedance measurements obtained by passing current between the intraluminal and subcutaneous return electrode. Histology of the colon was correlated with impedance measurements. The impedance of minimally inflamed (normal) tissue was 1.5–1.9 kΩ. Following TNBS injection, impedance significantly decreased within the inflammatory penumbra ( p < 0.05), and decreased more in the inflammatory epicentre ( p = 0.02). Histological damage correlated with impedance values ( p < 0.05). Thus, impedance values of 1.5–1.9, 1.3–1.4 and 0.9–1.1 kΩ corresponded to minimally inflamed, mildly inflamed and moderately inflamed tissue, respectively. In conclusion, transmural impedance is an objective, spatially localized biomarker of mucosal integrity, and distinguishes between severities of intestinal inflammation.en_US
dc.description.sponsorshipThis work was sponsored by the Defense Advanced Research Projects Agency (DARPA) BTO under the auspices of Dr Doug Weber and Dr Eric Van Gieson through the Space and Naval Warfare Systems Center (contract no. N66001-15-2-4060). The Bionics Institute and the Florey Institute of Neuroscience and Mental Health acknowledge the support they receive from the Victorian Government through its Operational Infrastructural Support Program.en_US
dc.identifier.citationPayne, S. C., J. Alexandrovics, R. Thomas, R. K. Shepherd, J. B. Furness, and J. B. Fallon. 2020. Transmural impedance detects graded changes of inflammation in experimental colitis. Royal Society Open Science. 7(2): 191819.en_US
dc.identifier.urihttp://repository.bionicsinstitute.org:8080/handle/123456789/386
dc.language.isoenen_US
dc.publisherThe Royal Society Publishingen_US
dc.subjectEndoscopeen_US
dc.subjectInflammatory bowel diseaseen_US
dc.subjectElectrical impedanceen_US
dc.subjectBiomarkersen_US
dc.subjectColitisen_US
dc.titleTransmural impedance detects graded changes of inflammation in experimental colitisen_US
dc.typeArticleen_US
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