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Title: Thermal damage threshold of neurons during infrared stimulation
Authors: Brown, William
Needham, Karina
Begeng, James
Thompson, Alexander
Nayagam, Bryony
Kameneva, Tatiana
Stoddart, Paul
Issue Date: Apr-2020
Publisher: Biomedical Optics Express
Citation: Brown, W. G. A., K. Needham, J. M. Begeng, A. C. Thompson, B. A. Nayagam, T. Kameneva, and P. R. Stoddart. 2020. Thermal damage threshold of neurons during infrared stimulation. Biomedical Optics Express. 11(4): 2224-2234.
Abstract: In infrared neural stimulation (INS), laser-evoked thermal transients are used to generate small depolarising currents in neurons. The laser exposure poses a moderate risk of thermal damage to the target neuron. Indeed, exogenous methods of neural stimulation often place the target neurons under stressful non-physiological conditions, which can hinder ordinary neuronal function and hasten cell death. Therefore, quantifying the exposure-dependent probability of neuronal damage is essential for identifying safe operating limits of INS and other interventions for therapeutic and prosthetic use. Using patch-clamp recordings in isolated spiral ganglion neurons, we describe a method for determining the dose-dependent damage probabilities of individual neurons in response to both acute and cumulative infrared exposure parameters based on changes in injection current. The results identify a local thermal damage threshold at approximately 60 °C, which is in keeping with previous literature and supports the claim that damage during INS is a purely thermal phenomenon. In principle this method can be applied to any potentially injurious stimuli, allowing for the calculation of a wide range of dose-dependent neural damage probabilities. Unlike histological analyses, the technique is well-suited to quantifying gradual neuronal damage, and critical threshold behaviour is not required.
URI: http://repository.bionicsinstitute.org:8080/handle/123456789/396
ISSN: 1741-2552
Appears in Collections:Other research publications

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