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Title: Combined optogenetic and electrical stimulation of auditory neurons increases effective stimulation frequency—an in vitro study
Authors: Hart, William
Richardson, Rachael
Kameneva, Tatiana
Thompson, Alex
Wise, Andrew
Fallon, James
Stoddart, Paul
Needham, Karina
Keywords: Optogenetics
Neural stimulation
Neuroprosthesis
Optical stimulation
Channelrhodopsin
Electrical stimulation
Patch clamp electrophysiology
Issue Date: Jan-2020
Publisher: IOP Publishing
Citation: Hart, W., R. Richardson, T. Kameneva, A. Thompson, A. K. Wise, J. B. Fallon, P. R. Stoddart, and K. Needham. 2020. Combined optogenetic and electrical stimulation of auditory neurons increases effective stimulation frequency - An in vitro study. Journal of Neural Engineering. 17(1): 016069.
Abstract: OBJECTIVE: The performance of neuroprostheses, including cochlear and retinal implants, is currently constrained by the spatial resolution of electrical stimulation. Optogenetics has improved the spatial control of neurons in vivo but lacks the fast-temporal dynamics required for auditory and retinal signalling. The objective of this study is to demonstrate that combining optical and electrical stimulation in vitro could address some of the limitations associated with each of the stimulus modes when used independently. APPROACH: The response of murine auditory neurons expressing ChR2-H134 to combined optical and electrical stimulation was characterised using whole cell patch clamp electrophysiology. MAIN RESULTS: Optogenetic costimulation produces a three-fold increase in peak firing rate compared to optical stimulation alone and allows spikes to be evoked by combined subthreshold optical and electrical inputs. Subthreshold optical depolarisation also facilitated spiking in auditory neurons for periods of up to 30 ms without evidence of wide-scale Na+ inactivation. Significance These findings may contribute to the development of spatially and temporally selective optogenetic-based neuroprosthetics and complement recent developments in "fast opsins".
URI: http://repository.bionicsinstitute.org:8080/handle/123456789/395
ISSN: 1741-2552
Appears in Collections:Bionic Hearing Research Publications

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