DSpace

About the Bionics Institute

 

Bionics Institute Research Online >
Other staff research publications >
Other research publications >


Title: Organotypic Cocultures of Human Pluripotent Stem Cell Derived- Neurons with Mammalian Inner Ear Hair Cells and Cochlear Nucleus Slices
Authors: Hyakumura, Tomoko
McDougall, Stuart
Finch, Sue
Needham, Karina
Dottori, Mirella
Nayagam, Bryony
Issue Date: Dec-2019
Publisher: Hindawi
Citation: Hyakumura, T., S. McDougall, S. Finch, K. Needham, M. Dottori, and B. A. Nayagam. 2019. Organotypic Cocultures of Human Pluripotent Stem Cell Derived-Neurons with Mammalian Inner Ear Hair Cells and Cochlear Nucleus Slices. Stem Cells International. 2019: 8419493.
Abstract: Stem cells have been touted as a source of potential replacement neurons for inner ear degeneration for almost two decades now; yet to date, there are few studies describing the use of human pluripotent stem cells (hPSCs) for this purpose. If stem cell therapies are to be used clinically, it is critical to validate the usefulness of hPSC lines in vitro and in vivo. Here, we present the first quantitative evidence that differentiated hPSC-derived neurons that innervate both the inner ear hair cells and cochlear nucleus neurons in coculture, with significantly more new synaptic contacts formed on target cell types. Nascent contacts between stem cells and hair cells were immunopositive for both synapsin I and VGLUT1, closely resembling expression of these puncta in endogenous postnatal auditory neurons and control cocultures. When hPSCs were cocultured with cochlear nucleus brainstem slice, significantly greater numbers of VGLUT1 puncta were observed in comparison to slice alone. New VGLUT1 puncta in cocultures with cochlear nucleus slice were not significantly different in size, only in quantity. This experimentation describes new coculture models for assessing auditory regeneration using well-characterised hPSC-derived neurons and highlights useful methods to quantify the extent of innervation on different cell types in the inner ear and brainstem.
URI: http://repository.bionicsinstitute.org:8080/handle/123456789/373
ISSN: 1687-966X (Print)
Appears in Collections:Other research publications

Files in This Item:

File Description SizeFormat
2019_Hyakumura_Organotypic.pdf5.08 MBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

DSpace Software Copyright © 2002-2010  Duraspace -