DSpace

About the Bionics Institute

 

Bionics Institute Research Online >
Bionic Hearing >
Bionic Hearing Research Publications >


Title: Firing frequency and entrainment maintained in primary auditory neurons in the presence of combined BDNF and NT3
Authors: Wright, Tess
Gillespie, Lisa
O'Leary, Stephen
Needham, Karine
Keywords: Cochle
Ion Channels in the nervous system
neurophysiology
Issue Date: 23-Jun-2016
Publisher: Macmillan Publishers Limited
Citation: Wright, T., Gillespie, L. N., O’Leary, S. J., & Needham, K. (2016). Firing frequency and entrainment maintained in primary auditory neurons in the presence of combined BDNF and NT3. Scientific Reports, 6, 28584.
Series/Report no.: 6;
Abstract: Primary auditory neurons rely on neurotrophic factors for development and survival. We previously determined that exposure to brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT3) alters the activity of hyperpolarization-activated currents (Ih) in this neuronal population. Since potassium channels are sensitive to neurotrophins, and changes in Ih are often accompanied by a shift in voltage-gated potassium currents (IK), this study examined IK with exposure to both BDNF and NT3 and the impact on firing entrainment during high frequency pulse trains. Whole-cell patch-clamp recordings revealed significant changes in action potential latency and duration, but no change in firing adaptation or total outward IK. Dendrotoxin-I (DTX-I), targeting voltage-gated potassium channel subunits KV1.1 and KV1.2, uncovered an increase in the contribution of DTX-I sensitive currents with exposure to neurotrophins. No difference in Phrixotoxin-1 (PaTX-1) sensitive currents, mediated by KV4.2 and KV4.3 subunits, was observed. Further, no difference was seen in firing entrainment. These results show that combined BDNF and NT3 exposure influences the contribution of KV1.1 and KV1.2 to the low voltage-activated potassium current (IKL). Whilst this is accompanied by a shift in spike latency and duration, both firing frequency and entrainment to high frequency pulse trains are preserved.
URI: http://repository.bionicsinstitute.org:8080/handle/123456789/209
Appears in Collections:Bionic Hearing Research Publications

Files in This Item:

File Description SizeFormat
2016 Wright et al. firing frequency - cover.pdf1.34 MBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

DSpace Software Copyright © 2002-2010  Duraspace -