Bionics Institute Research Online >
Other staff research publications >
Other research publications >
|Title: ||Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells|
|Authors: ||Hernandez, Damian|
|Issue Date: ||2016|
|Publisher: ||Hindawi Publishing Corporation|
|Citation: ||Hernández, D., R. Millard, P. Sivakumaran, R. Ching-Bong Wong, D. E. Crombie, A. W. Hewitt, H. Liang, S. S. C. Hung, A. Pébay, R. K. Shepherd, G. J. Dusting & S. Y. Lim (2016). Electrical stimulation promotes cardiac differentiation of human induced pluripotent stem cells. Stem Cells International, 2016|
|Abstract: ||Background.Human induced pluripotent stemcells (iPSCs) are an attractive source of cardiomyocytes for cardiac repair and regeneration.
In this study, we aim to determine whether acute electrical stimulation of human iPSCs can promote their differentiation
to cardiomyocytes. Methods. Human iPSCs were differentiated to cardiac cells by forming embryoid bodies (EBs) for 5 days. EBs
were then subjected to brief electrical stimulation and plated down for 14 days. Results. In iPS(Foreskin)-2 cell line, brief electrical
stimulation at 65mV/mm or 200mV/mm for 5 min significantly increased the percentage of beating EBs present by day 14 after
plating. Acute electrical stimulation also significantly increased the cardiac gene expression of ACTC1, TNNT2, MYH7, and MYL7.
However, the cardiogenic effect of electrical stimulation was not reproducible in another iPS cell line,CERA007c6. Beating EBs from
control and electrically stimulated groups expressed various cardiac-specific transcription factors and contractile muscle markers.
Beating EBs were also shown to cycle calcium and were responsive to the chronotropic agents, isoproterenol and carbamylcholine,
in a concentration-dependent manner. Conclusions. Our results demonstrate that brief electrical stimulation can promote cardiac
differentiation of human iPS cells.The cardiogenic effect of brief electrical stimulation is dependent on the cell line used.|
|Appears in Collections:||Other research publications|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.